Ues for the amount of antibody bound were obtained by subtracting binding measured in the absence of sCD4 from the binding measured at each time point after the sCD4 pulse.buffer, cells were incubated at room temperature for 45 minutes in FACS buffer containing a phycoerythrin-conjugated goat antihuman IgG antibody (Sigma) and an anti-CD4 fluoresceinconjugated antibody (OKT4, eBioscience) to measu