Ruffles and C3bi-particle binding. DIC images were captured at 10-s intervals shortly after particle addition in both PMA- and LPS-activated RAW264.7 cells. Live imaging revealed prominent membrane ruffles that formed spontaneously on the dorsal surface of activated, but not on control cells (Figure 2 A, also see Supplementary Movie S2A, control). Excess C3bi-sRBCs were added, and particles thatVo
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