Bled into a complex with E1 and/or E3 or that an intermediate protein was required. This led to testing of the B. subtilis glycine cleavage H protein (GcvH) because (as noted above) it is essentially a lipoyl domain and is not part of a stable complex. B. subtilis GcvH turned out to readily accept octanoate either from octanoyl-ACP or from octanoic acid plus ATP in reactions catalyzed by the B. su