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Ipt Author Manuscript Author Manuscript Author ManuscriptExtended Data Figure 2. Development of anti-MAb immune responses in recipients of antiHIV-1 bNAbsNature. Author manuscript; available in PMC 2016 November 29.Gautam et al.Pagea, b, c, d, Longitudinal analysis of anti-VRC01, anti-3BNC117, anti-10-1074 and antiVRC01-LS antibody responses respectively, following a single intravenous infusion of

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Infection in vitro, this protein was tested for clinical efficacy in HIV-1-infected individuals; however, no effect on plasma viral loads was observed [13]. Further examination revealed that doses of sCD4 that were significantly higher than those achieved in the clinical trial were required to neutralize primary clinical isolates of HIV-1, in contrast to the relatively sensitive, laboratory-adapte

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Condition. In total,54 sample injections (12 fetuin glycoprobe elutions, 24 standard glycoprobe elutions and 18 blank elutions) per each sperm condition (CM and NCM) were performed for MS/MS analysis of CREDEX experiments. In order to minimize instrumental variability, a defined batch file was programmed for sample injection. Specifically, samples were injected in groups of nine including the six

T 1500 rpm for 6 min at 4 , washed twice with 2 mL PBS + 2 BSA, and resuspended in 50 of PBS + 2 BSA solution for blocking. Antibodies were added to corresponding tubes and incubated in the dark at 4 for 45 min. Cells were removed from incubation, washed again twice with PBS + 2 BSA, and resuspended in PBS containing 0.5 formaldehyde. Cell populations were gated on a minimum of 250,000 ce