Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup
S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup