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Ulates expression of CD40 receptor on the surface of endothelial cells and the shedding of the ligand [110]. Inhibition of CD40-CD40L interactions was shown to retard the development of experimental autoimmune encephalomyelitis (EAE), in an animal model of MS [111]. In vitro studies demonstrated IFNg, which is overexpressed in the HIV infected brain [112] up-regulates the expression of CD40 by mic

Ulate CNS inflammation at the level of the BBB. High levels of soluble CD40L in CSF and plasma of HIV-infected patients with cognitive impairment has been demonstrated as well. Exposure of primary human brain microvascular endothelial cells (BMVECs) to CD40L increased the expression of adhesion molecules intracellular adhesion molecule-1 and vascular cell adhesion molecule-1, which yielded a fourf

Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a

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