http://64188.org/story.php?title=washing-buffer-140-mm-nacl-1-8-mm-cacl2-1-mm-mgcl2-and-20-mm-tris-6 Washing buffer (140 mM NaCl, 1.8 mM CaCl2, 1 mM MgCl2 and 20 mM Tris, pH 7.5). A horseradish peroxidase-conjugated antibody specific for the Fc region of human IgG was then incubated with the samples for 45 minutes at room temperature. Cells were washed 5 times with blocking buffer and 5 times with washing buffer. HRP enzyme activity was determined after the addition of 33 ml per well of a 1:1 mix Fri, 05 May 2023 11:34:20 UTC en