http://64188.org/story.php?title=ogenized-and-centrifuged-at-1000-g-and-the-supernatant-was-removed-for Ogenized and centrifuged at 1000 g, and the supernatant was removed. For measurement of forskolinstimulated cAMP in lysate, we added 100 ?00 g of tissue to incubation buffer containing 50 mM ATP, 30 M IBMX, and 5 mM creatine phosphate with and without forskolin (50 M). We incubated the mixture for 10 min at 37 in a water bath and stopped the reaction by adding 5 TCA, which was subsequently extra Fri, 31 Mar 2023 06:44:23 UTC en